MASTURBATING DECREASES TESTOSTERONE
“These effects were observed both before and after sexual abstinence. In contrast, although plasma testosterone was unaltered by orgasm, higher testosterone concentrations were observed following the period of abstinence. These data demonstrate that acute abstinence does not change the neuroendocrine response to orgasm but does produce elevated levels of testosterone in males.”
Source: https://pubmed.ncbi.nlm.nih.gov/11760788/